Overview

  • Product name
  • Description
    Rabbit polyclonal to SAE1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 3-217 of Human SAE1 (AAH00344)

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells, A549 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab97523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function
    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Tissue specificity
    Expression level increases during S phase and drops in G2 phase (at protein level).
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of SUMO1 antibody
    • AOS1 antibody
    • HSPC140 antibody
    • Sae1 antibody
    • SAE1_HUMAN antibody
    • Sentrin/SUMO activating protein AOS1 antibody
    • SUA1 antibody
    • SUMO 1 activating enzyme E1 N subunit antibody
    • SUMO 1 activating enzyme subunit 1 antibody
    • SUMO-activating enzyme subunit 1 antibody
    • Ubiquitin like protein SUMO1 activating enzyme antibody
    • Ubiquitin-like 1-activating enzyme E1A antibody
    • UBL E1A antibody
    • UBLE1A antibody
    see all

Images

  • All lanes : Anti-SAE1 antibody (ab97523) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 38 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97523 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using ab97523 at 1/100 dilution.

References

ab97523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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