Overview

  • Product nameAnti-SAE1 antibody
    See all SAE1 primary antibodies
  • Description
    Rabbit polyclonal to SAE1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 3-217 of Human SAE1 (AAH00344)

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells, A549 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab97523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator of SUMO1 antibody
    • AOS1 antibody
    • HSPC140 antibody
    • Sae1 antibody
    • SAE1_HUMAN antibody
    • Sentrin/SUMO activating protein AOS1 antibody
    • SUA1 antibody
    • SUMO 1 activating enzyme E1 N subunit antibody
    • SUMO 1 activating enzyme subunit 1 antibody
    • SUMO-activating enzyme subunit 1 antibody
    • Ubiquitin like protein SUMO1 activating enzyme antibody
    • Ubiquitin-like 1-activating enzyme E1A antibody
    • UBL E1A antibody
    • UBLE1A antibody
    see all

Anti-SAE1 antibody images

  • All lanes : Anti-SAE1 antibody (ab97523) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 38 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97523 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using ab97523 at 1/100 dilution.

References for Anti-SAE1 antibody (ab97523)

ab97523 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97523.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"