Overview

  • Product nameAnti-SAE2 / UBA2 antibody [4A3]
    See all SAE2 / UBA2 primary antibodies
  • Description
    Mouse monoclonal [4A3] to SAE2 / UBA2
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human, Monkey
    Predicted to work with: Dog
  • Immunogen

    Recombinant full length Human SAE2/ UBA2 produced in HEK293T cells (NP_005490).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY SAE2 / UBA2 cDNA; A549, COS7, HeLa, HepG2, HT29, Jurkat, MCF7, MDCK and PC12 cell extracts; COS7 cells transiently transfected with pCMV6-ENTRY SAE2 / UBA2 cDNA; Human endometrium, lymphoma, prostate and Human prostate carcinnoma tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab118404 is purified from Mouse ascites fluid by affinity chromatography.
  • Clonality Monoclonal
  • Clone number4A3
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab118404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 71 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anthracycline associated resistance ARX antibody
    • Anthracycline-associated resistance ARX antibody
    • ARX antibody
    • FLJ13058 antibody
    • HRIHFB2115 antibody
    • SAE 2 antibody
    • SAE2 antibody
    • SAE2_HUMAN antibody
    • SUMO 1 activating enzyme subunit 2 antibody
    • SUMO activating enzyme subunit 2 antibody
    • SUMO-activating enzyme subunit 2 antibody
    • UBA2 antibody
    • UBA2 ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin like 1 activating enzyme E1B antibody
    • Ubiquitin like modifier activating enzyme 2 antibody
    • Ubiquitin-like 1-activating enzyme E1B antibody
    • UBLE1B antibody
    see all

Anti-SAE2 / UBA2 antibody [4A3] images

  • All lanes : Anti-SAE2 / UBA2 antibody [4A3] (ab118404) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLA cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201288)
  • All lanes : Anti-SAE2 / UBA2 antibody [4A3] (ab118404) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SAE2 / UBA2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 71 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201288)
  • ab118404 at 1/100 dilution staining SAE2 / UBA2 in COS7 cells transiently transfected by pCMV6-ENTRY SAE2 / UBA2 by Immunofluorescence.
  • ab118404 at 1/150 dilution staining SAE2 / UBA2 in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab118404 at 1/150 dilution staining SAE2 / UBA2 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab118404 at 1/50 dilution staining SAE2 / UBA2 in Paraffin-embedded Human Prostate carcinoma tissue by Immunohistochemistry.
  • ab118404 at 1/150 dilution staining SAE2 / UBA2 in Paraffin-embedded Human Lymphoma tissue by Immunohistochemistry.

References for Anti-SAE2 / UBA2 antibody [4A3] (ab118404)

ab118404 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"