Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-SAE2 / UBA2 antibody [EPR14880]
See all SAE2 / UBA2 primary antibodies -
DescriptionRabbit monoclonal [EPR14880] to SAE2 / UBA2
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, IP, ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SAE2/ UBA2 aa 50-150. The exact sequence is proprietary.
Database link: Q9UBT2 -
Positive control
- HeLa, A549, K562, Raw264.7 and PC-12 cell lysates; Human colon tissue; Mouse brain tissue; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR14880
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab185955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. | |
| IP | 1/30 - 1/50. | |
| ICC/IF | 1/100. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 71 kDa). |
Target
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FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
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PathwayProtein modification; protein sumoylation.
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Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 10054 Human
- Entrez Gene: 50995 Mouse
- Entrez Gene: 308508 Rat
- Omim: 613295 Human
- SwissProt: Q9UBT2 Human
- SwissProt: Q9Z1F9 Mouse
- Unigene: 631580 Human
- Unigene: 27560 Mouse
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Alternative names
- Anthracycline associated resistance ARX antibody
- Anthracycline-associated resistance ARX antibody
- ARX antibody
see all
Images
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![Western blot - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)](http://a.abcam.com/ps/products/185/ab185955/Images/ab185955-219603-ab185955WB.jpg)
Western blot - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa (why is the actual band size different from the predicted?) -
![Western blot - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)](http://a.abcam.com/ps/products/185/ab185955/Images/ab185955-219602-ab185955WBb.jpg)
Western blot - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)](http://a.abcam.com/ps/products/185/ab185955/Images/ab185955-219601-ab185955IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)](http://a.abcam.com/ps/products/185/ab185955/Images/ab185955-219600-ab185955IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
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![Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)](http://a.abcam.com/ps/products/185/ab185955/Images/ab185955-219599-ab185955IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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![Immunoprecipitation - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)](http://a.abcam.com/ps/products/185/ab185955/Images/ab185955-219598-ab185955IP.jpg)
Immunoprecipitation - Anti-SAE2 / UBA2 [EPR14880] antibody (ab185955)Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with ab185955 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
References
ab185955 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"