The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Anthracycline associated resistance ARX
Anthracycline-associated resistance ARX
SUMO 1 activating enzyme subunit 2
SUMO activating enzyme subunit 2
SUMO-activating enzyme subunit 2
UBA2 ubiquitin activating enzyme E1 homolog
Ubiquitin like 1 activating enzyme E1B
Ubiquitin like modifier activating enzyme 2
Ubiquitin-like 1-activating enzyme E1B
FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.