Anti-SAFB antibody [4i59] (ab18086)

Overview

  • Product nameAnti-SAFB antibody [4i59]
    See all SAFB primary antibodies
  • Description
    Mouse monoclonal [4i59] to SAFB
  • SpecificityAb18086 recognizes HET/SAFB, Mr 130kDa; and a protein, Mr 34kDa.
  • Tested applicationsSuitable for: WB, IP, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide:

    CPEARDKEDGRKF

    , corresponding to amino acids 345 to 357 of SAFB.

  • EpitopeThe immunizing sequence has 8/11 identical amino acids in mouse and human transcription factor tbx2 (T-box protein 2).
  • Positive control
    • An independent laboratory has shown HET/SAFB to be immunoprecipitated from MCF-7 and HepG2 cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number4i59
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 102 kDa.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionBinds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
  • Tissue specificityUbiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779C1727 antibody
    • glutathione S transferase fusion protein antibody
    • HAP antibody
    • HET antibody
    • HSP27 ERE TATA binding protein antibody
    • HSP27 ERE-TATA-binding protein antibody
    • HSP27 estrogen response element-TATA box-binding protein antibody
    • SAF B antibody
    • SAF-B antibody
    • SAF-B1 antibody
    • SAFB 1 antibody
    • SAFB antibody
    • SAFB1 antibody
    • SAFB1_HUMAN antibody
    • Scaffold attachment factor B1 antibody
    see all

References for Anti-SAFB antibody [4i59] (ab18086)

ab18086 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I can tell you that the tissue tested in IHC was the human cell line MCF-7 human breast adenocarcinoma cell line and also the human hepatocellular liver carcinoma cell line HepG2. I have no information available on an ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"