Overview

  • Product nameAnti-SAFB antibody
    See all SAFB primary antibodies
  • Description
    Rabbit polyclonal to SAFB
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 476-705 of Human SAFB (NP_002958).

  • Positive control
    • A431, H1299, HeLa and HepG2 cell lysates for WB; HeLa cells for ICC/IF

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 103 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionBinds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
  • Tissue specificityUbiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779C1727 antibody
    • glutathione S transferase fusion protein antibody
    • HAP antibody
    • HET antibody
    • HSP27 ERE TATA binding protein antibody
    • HSP27 ERE-TATA-binding protein antibody
    • HSP27 estrogen response element-TATA box-binding protein antibody
    • SAF B antibody
    • SAF-B antibody
    • SAF-B1 antibody
    • SAFB 1 antibody
    • SAFB antibody
    • SAFB1 antibody
    • SAFB1_HUMAN antibody
    • Scaffold attachment factor B1 antibody
    see all

Anti-SAFB antibody images

  • Anti-SAFB antibody (ab97520) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 103 kDa
  • ab97520, at a 1/100 dilution, staining SAFB in paraformaldehyde fixed HeLa cells by Immunofluorescence analysis.
    Lower image was merged with DNA probe.

References for Anti-SAFB antibody (ab97520)

ab97520 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"