Recombinant
RabMAb

Anti-SAFB antibody [EPR13588] - C-terminal (ab187650)

Overview

  • Product name
    Anti-SAFB antibody [EPR13588] - C-terminal
    See all SAFB primary antibodies
  • Description
    Rabbit monoclonal [EPR13588] to SAFB - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SAFB aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15424

  • Positive control
    • HeLa and A431 cells, SH SY5Y and 293 cell line lysates, mouse heart tissue; Human and rat brain tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13588
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 103 kDa).
ICC/IF 1/100.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation.
  • Tissue specificity
    Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779C1727 antibody
    • glutathione S transferase fusion protein antibody
    • HAP antibody
    • HET antibody
    • HSP27 ERE TATA binding protein antibody
    • HSP27 ERE-TATA-binding protein antibody
    • HSP27 estrogen response element-TATA box-binding protein antibody
    • SAF B antibody
    • SAF-B antibody
    • SAF-B1 antibody
    • SAFB 1 antibody
    • SAFB antibody
    • SAFB1 antibody
    • SAFB1_HUMAN antibody
    • Scaffold attachment factor B1 antibody
    see all

Images

  • All lanes : Anti-SAFB antibody [EPR13588] - C-terminal (ab187650) at 1/20000 dilution

    Lane 1 : HeLa cell line lysate
    Lane 2 : SH SY5Y cell line lysate
    Lane 3 : 293 cell line lysate
    Lane 4 : A431 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 150 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-SAFB antibody [EPR13588] - C-terminal (ab187650) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat brain tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 103 kDa
    Observed band size: 150 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling SAFB with ab187650 at 1/500 followed by ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hemotoxylin.

  • Immunohistochemical analysis of paraffin embedded rat brain tissue labeling SAFB with ab187650 at 1/500 followed by ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG and counterstained with Hemotoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling SAFB with ab187650 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counterstained with Dapi (blue).

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling SAFB with ab187650 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counterstained with Dapi (blue).

References

ab187650 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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