Overview

  • Product nameAnti-SALL1 antibody
    See all SALL1 primary antibodies
  • Description
    Rabbit polyclonal to SALL1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Chicken, Human, Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.

    (Peptide available as ab31903.)

  • Positive control
    • The antibody gave a positive signal in E14Tg2a cell lysate. The antibody gave a positive signal in the following methanol fixed cell line: Mouse embryonic stem cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).

Target

  • FunctionTranscriptional repressor involved in organogenesis.
  • Tissue specificityHighest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
  • Involvement in diseaseDefects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
    Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
  • Sequence similaritiesBelongs to the sal C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
  • Developmental stageIn fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSal1 antibody
    • Sal like protein 1 antibody
    • Sal-1 antibody
    • Sal-like protein 1 antibody
    • Sal1 antibody
    • SALL1 antibody
    • SALL1_HUMAN antibody
    • Spalt-like transcription factor 1 antibody
    • TBS antibody
    • Zinc finger protein 794 antibody
    • Zinc finger protein SALL1 antibody
    • Zinc finger protein Spalt-1 antibody
    see all

Anti-SALL1 antibody images

  • Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744

  • ab31526 staining SALL1 in metanephric blastema cells, murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab31526 stained Mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31526 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SALL1 antibody (ab31526)

This product has been referenced in:
  • Zhou Q  et al. Establishment of a proteome profile and identification of molecular markers for mouse spermatogonial stem cells. J Cell Mol Med 19:521-34 (2015). IF ; Mouse . Read more (PubMed: 25352495) »
  • Bagherie-Lachidan M  et al. Stromal Fat4 acts non-autonomously with Dchs1/2 to restrict the nephron progenitor pool. Development 142:2564-73 (2015). Read more (PubMed: 26116661) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Fruit fly (Drosophila melanogaster) Tissue (imaginal disc)
Specification imaginal disc
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Verified customer

Submitted Sep 03 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
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Verified customer

Submitted Dec 17 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"