Overview

  • Product name
  • Description
    Rabbit polyclonal to SALL1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Chicken, Human, Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.

    (Peptide available as ab31903.)

  • Positive control
    • The antibody gave a positive signal in E14Tg2a cell lysate. The antibody gave a positive signal in the following methanol fixed cell line: Mouse embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab31526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).

Target

  • Function
    Transcriptional repressor involved in organogenesis.
  • Tissue specificity
    Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
  • Involvement in disease
    Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
    Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
  • Sequence similarities
    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
  • Developmental stage
    In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSal1 antibody
    • Sal like protein 1 antibody
    • Sal-1 antibody
    • Sal-like protein 1 antibody
    • Sal1 antibody
    • SALL1 antibody
    • SALL1_HUMAN antibody
    • Spalt-like transcription factor 1 antibody
    • TBS antibody
    • Zinc finger protein 794 antibody
    • Zinc finger protein SALL1 antibody
    • Zinc finger protein Spalt-1 antibody
    see all

Anti-SALL1 antibody images

  • Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744

  • ab31526 staining SALL1 in metanephric blastema cells, murine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab31526 stained Mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31526 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SALL1 antibody (ab31526)

This product has been referenced in:
  • Reginensi A  et al. A critical role for NF2 and the Hippo pathway in branching morphogenesis. Nat Commun 7:12309 (2016). IF . Read more (PubMed: 27480037) »
  • Kumar N  et al. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. Elife 4:N/A (2015). Read more (PubMed: 26554899) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Frontal cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Frontal cortex
Blocking step
FBS as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (imaginal disc)
Specification
imaginal disc
Username

Abcam user community

Verified customer

Submitted Sep 03 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

Submitted Dec 17 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up