Anti-SALL1 antibody (ab31526)
Key features and details
- Rabbit polyclonal to SALL1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SALL1 antibody
See all SALL1 primary antibodies -
Description
Rabbit polyclonal to SALL1 -
Host species
Rabbit -
Specificity
Replenishment batches of ab31526 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Chicken, Human, Drosophila melanogaster -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.
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Positive control
- ICC/IF: mES cells. WB: E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa). |
Target
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Function
Transcriptional repressor involved in organogenesis. -
Tissue specificity
Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver. -
Involvement in disease
Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation. -
Sequence similarities
Belongs to the sal C2H2-type zinc-finger protein family.
Contains 9 C2H2-type zinc fingers. -
Developmental stage
In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395446 Chicken
- Entrez Gene: 6299 Human
- Entrez Gene: 58198 Mouse
- Omim: 602218 Human
- SwissProt: Q9NSC2 Human
- SwissProt: Q9ER74 Mouse
- Unigene: 135787 Human
- Unigene: 214361 Mouse
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Alternative names
- HSal1 antibody
- Sal like protein 1 antibody
- Sal-1 antibody
see all
Images
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Anti-SALL1 antibody (ab31526) at 1 µg/ml + Mouse embryonic stem cell lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 140 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesBlocking buffer: 2% BSA block
Gel type: TA
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ab31526 staining SALL1 in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31526 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesAlthough the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab31526 has been referenced in 15 publications.
- Lavicky J et al. The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium. J Bone Miner Res 37:323-339 (2022). PubMed: 34783080
- Scott EP et al. The zinc finger transcription factor Sall1 is required for the early developmental transition of microglia in mouse embryos. Glia 70:1720-1733 (2022). PubMed: 35567352
- Errede M et al. Microglia-derived CCL2 has a prime role in neocortex neuroinflammation. Fluids Barriers CNS 19:68 (2022). PubMed: 36042496
- Krivanek J et al. Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth. Nat Commun 11:4816 (2020). PubMed: 32968047
- Chi D et al. Spalt-Like Transcription Factor 1 (SALL1) Gene Expression Inhibits Cell Proliferation and Cell Migration of Human Glioma Cells Through the Wnt/ß-Catenin Signaling Pathway. Med Sci Monit Basic Res 25:128-138 (2019). PubMed: 31040265