Baculovirus-expressed recombinant fragment, corresponding to amino acids 258/499 of Human SALL1
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab31526 as a replacement.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium Azide
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ab41974 was purified by Ammonium sulfate fractionation.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.
Transcriptional repressor involved in organogenesis.
Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
Involvement in disease
Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss. Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
Belongs to the sal C2H2-type zinc-finger protein family. Contains 9 C2H2-type zinc fingers.
In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SALL1 antibody [K9814] (ab41974)Image courtesy of an anonymous Abreview
Ab41974 staining SALL1 in Mouse embryonic kidney at tissue sections by Immunohistochemistry (IHC- P- paraformaldehyde-fixed, paraffin- embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10µg/ml BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a Tris/EDTA (pH9). Samples were incubated with primary antibody (1/100 with 6% Horse serum in PBST) for 16hours at 4oC. An Alexa Fluor® 568 donkey anti-mouse polyclonal (1/500 dilution) was used as the secondary antibody.