Anti-SALL1 antibody [K9814] (ab41974)

Overview

  • Product name
    Anti-SALL1 antibody [K9814]
    See all SALL1 primary antibodies
  • Description
    Mouse monoclonal [K9814] to SALL1
  • Specificity
    This antibody is specific for SALL1.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Baculovirus-expressed recombinant fragment, corresponding to amino acids 258/499 of Human SALL1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab31526 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab41974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.

Target

  • Function
    Transcriptional repressor involved in organogenesis.
  • Tissue specificity
    Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.
  • Involvement in disease
    Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
    Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.
  • Sequence similarities
    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
  • Developmental stage
    In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSal1 antibody
    • Sal like protein 1 antibody
    • Sal-1 antibody
    • Sal-like protein 1 antibody
    • Sal1 antibody
    • SALL1 antibody
    • SALL1_HUMAN antibody
    • Spalt-like transcription factor 1 antibody
    • TBS antibody
    • Zinc finger protein 794 antibody
    • Zinc finger protein SALL1 antibody
    • Zinc finger protein Spalt-1 antibody
    see all

Images

  • Ab41974 staining SALL1 in Mouse embryonic kidney at tissue sections by Immunohistochemistry (IHC- P- paraformaldehyde-fixed, paraffin- embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10µg/ml BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a Tris/EDTA (pH9).  Samples were incubated with primary antibody (1/100 with 6% Horse serum in PBST) for 16hours at 4oC. An Alexa Fluor® 568 donkey anti-mouse polyclonal (1/500 dilution) was used as the secondary antibody.

    See Abreview

References

ab41974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse embryonic kidney at 13.5 dpc)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
Permeabilization
No
Specification
Mouse embryonic kidney at 13.5 dpc
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Sep 16 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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