Anti-Sall4 antibody (ab31968)

Overview

  • Product nameAnti-Sall4 antibody
    See all Sall4 primary antibodies
  • Description
    Rabbit polyclonal to Sall4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse Sall4.

    (Peptide available as ab30772.)

  • Positive control
    • This antibody gave a positive signal in E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 113, 66 kDa). We believe this 140 kDa band corresponds to the Sall4a isoform.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionProbable transcription factor.
  • Tissue specificityExpressed in testis.
  • Involvement in diseaseDefects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
    Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.
  • Sequence similaritiesBelongs to the sal C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA407717 antibody
    • AL022809 antibody
    • AW536104 antibody
    • C330011P20Rik antibody
    • C78083 antibody
    • C78563 antibody
    • dJ1112F19.1 antibody
    • DRRS antibody
    • HSAL4 antibody
    • Sal like 4 (Drosophila) antibody
    • Sal like 4 antibody
    • Sal like Protein 4 antibody
    • Sal-like protein 4 antibody
    • Sall4 antibody
    • SALL4_HUMAN antibody
    • Spalt like transcription factor 4 antibody
    • Tex20 antibody
    • Zinc finger protein 797 antibody
    • Zinc finger protein SALL4 antibody
    • ZNF797 antibody
    see all

Anti-Sall4 antibody images

  • Anti-Sall4 antibody (ab31968) at 1/1 dilution + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 113, 66 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    This antibody detects a band at ~140kDa, which is believed to be the Sall4a isoform.
  • All lanes : Anti-Sall4 antibody (ab31968) at 1/1000 dilution

    Lane 1 : Mouse ES Cell Lysate
    Lane 2 : Mouse ES Cell Lysate
    Lane 3 : Mouse ES Cell Lysate

    Lysates/proteins at 14 µg per lane.


    Predicted band size : 113, 66 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa (possible isoform,non-specific binding),70 kDa (possible isoform,non-specific binding),85 kDa (possible isoform,non-specific binding).

    This image is courtesy of S. Rao, Harvard University

    The image shows detection of a Sall4 band at 145 kDa. Sall4 is known to run at a higher molecular weight than predicted by its primary amino acid sequence. The lower molecular weight bands may represent isoforms or non-specific binding.
  • ICC/IF image of ab31968 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31968, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

References for Anti-Sall4 antibody (ab31968)

ab31968 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Stem cells)
Loading amount 200000 cells
Specification Mouse Embryonic Stem cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Joe Segal

Verified customer

Submitted Jan 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic Stem Cells (D3))
Specification Embryonic Stem Cells (D3)
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 21°C
Username

Joe Segal

Verified customer

Submitted Sep 24 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - other (male germ cell culture)
Specification male germ cell culture
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Microarray
Positive control Mouse germ cell cultures are positive for Sall4 by ICC, western blot and RT-PCR. Pull-down with this antibody was performed with cultured mouse male germ cells (>75% expressing cells). 30ug chromatin and 5ug antibody used per ChIP reaction.
Negative control Input - gDNA from chromatin from same sample.
Username

Abcam user community

Verified customer

Submitted Jan 04 2012

DISCOUNT CODES: *** Expiration date: April 16, 2012 These codes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in ChIP and include the corresponding code in the “...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells)
Loading amount 40 µg
Specification Mouse Embryonic Stem Cells
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Aug 31 2011

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
Loading amount 25 µg
Specification Mouse embryonic stem cell
Gel Running Conditions Reduced Denaturing (8% Bis-Tris gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"