Anti-Sall4 antibody (ab61703)

Overview

  • Product nameAnti-Sall4 antibody
    See all Sall4 primary antibodies
  • Description
    Goat polyclonal to Sall4
  • SpecificityThis antibody is expected to recognize isoform a (NP_780512.2) and isoform b (NP_958797.2).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    C-DQSKKGKGKPQN

    , corresponding to internal sequence amino acids 355-366 of Mouse Sall4 according to NP_780512.2.

  • Positive control
    • Rat Testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab61703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).

Target

  • FunctionProbable transcription factor.
  • Tissue specificityExpressed in testis.
  • Involvement in diseaseDefects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
    Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.
  • Sequence similaritiesBelongs to the sal C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA407717 antibody
    • AL022809 antibody
    • AW536104 antibody
    • C330011P20Rik antibody
    • C78083 antibody
    • C78563 antibody
    • dJ1112F19.1 antibody
    • DRRS antibody
    • HSAL4 antibody
    • Sal like 4 (Drosophila) antibody
    • Sal like 4 antibody
    • Sal like Protein 4 antibody
    • Sal-like protein 4 antibody
    • Sall4 antibody
    • SALL4_HUMAN antibody
    • Spalt like transcription factor 4 antibody
    • Tex20 antibody
    • Zinc finger protein 797 antibody
    • Zinc finger protein SALL4 antibody
    • ZNF797 antibody
    see all

Anti-Sall4 antibody images

  • Anti-Sall4 antibody (ab61703) at 0.1 µg/ml + Rat Testis lysate (35µg protein in RIPA buffer).

    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Additional bands at : 24 kDa,28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Sall4 antibody (ab61703)

ab61703 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - other (mouse germ line stem cell cultures)
Specification mouse germ line stem cell cultures
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Microarray
Positive control We have previously shown by ICC, western blot and RT-PCR that Sall4 is present in this sample. Pull-down with this antibody was performed with cultured mouse male germ cells (>75% expressing cells). 30ug chromatin and 5ug antibody used per ChIP reaction.
Negative control Input - gDNA from chromatin from same sample.
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Submitted Jan 04 2012

DISCOUNT CODES: *** Expiration date: April 16, 2012 These codes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in ChIP and include the corresponding code in the “...

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