Anti-Salmonella 0-12 A, B, D group antibody [10D9H] (ab8272)

Overview

  • Product name
    Anti-Salmonella 0-12 A, B, D group antibody [10D9H]
    See all Salmonella 0-12 A, B, D group primary antibodies
  • Description
    Mouse monoclonal [10D9H] to Salmonella 0-12 A, B, D group
  • Specificity
    This antibody reacts with A,B,D-group 0-12 antigen
  • Tested applications
    Suitable for: Dot blot, ELISA, ICC/IFmore details
  • Immunogen

    Heat inactivated bacterial cells of Salmonella enteriditis,paratyphi and typhimurium

  • General notes
    Binding constants: LPS A = 2.1 x 109 LPS B = 1.1 x 109 LPS D = 6.5 x 108 LPS E = 1.0 x 106


    This antibody detects Salmonella enteriditis D, paratyphi A and typhimurium B

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This antibody detects Salmonella enteriditis D, paratyphi A and typhimurium B
  • Clonality
    Monoclonal
  • Clone number
    10D9H
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

References

ab8272 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This antibody has been tested in dot-blot, ELISA and immunofluorescence.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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