Anti-Salmonella antibody [B343M] (ab72989)

Overview

  • Product nameAnti-Salmonella antibody [B343M]
    See all Salmonella primary antibodies
  • Description
    Mouse monoclonal [B343M] to Salmonella
  • Specificityab72989 is specific for the common LPS core of Salmonella.
  • Tested applicationsSuitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with all Salmonella O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1 and G2. Does not react with E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus and Legionella.
  • Immunogen

    Mixture of 4 Salmonella serotypes (whole organisms).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure.
  • ClonalityMonoclonal
  • Clone numberB343M
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution. LPS extraction recommended.
    ICC/IF: Use at an assay dependent dilution.


    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

    References for Anti-Salmonella antibody [B343M] (ab72989)

    ab72989 has not yet been referenced specifically in any publications.

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    I am pleased to now have further information for you. We would recommend to use the following steps in this case:

    Add the resin to the 1 ml of antibody in a 1.5 ml eppendorf. Then ...

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    I am sorry to confirm we would not recommend to try conjugating an antibody with the Easy Link kit if it has a concentration less than 1mg/ml. We would not be able to guarantee the efficiency of the conjugation. <...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"