Anti-Salmonella antibody (Biotin) (ab69255)

Overview

  • Product name
    Anti-Salmonella antibody (Biotin)
    See all Salmonella primary antibodies
  • Description
    Rabbit polyclonal to Salmonella (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab69255 reacts with Salmonella sp. in bacterial and infected tissue samples.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with Salmonella sp.
  • Immunogen

    Mixture of Salmonella enteriditis, Salmonella typhimurium and Salmonella heidelburg (O and H antigens).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

References

ab69255 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Bacteria Cell (Salmonella infected liver)
Specification
Salmonella infected liver
Permeabilization
Yes - 0.25% Triton X-100
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Mar 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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