Overview

  • Product nameAnti-Salmonella antibody (FITC)
    See all Salmonella primary antibodies
  • Description
    Rabbit polyclonal to Salmonella (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityPolyvalent for Salmonella "O" & "H" antigens. Antiserum is not absorbed and does react with related Enterobacteriaceae.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with Salmonella species. Not yet tested in other species.
  • Immunogen

    Tissue/ cell preparation (Mixture of S. enteriditis, S. typhimurium, and S. heidelburg).

  • General notes

    Purified IgG fraction of the antiserum covalent coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notesIF: This antibody has been tested in IFA using organisms fixed on slides. After the organism suspension is spotted on glass slides and air dried, the slides are fixed by immersion in 100% acetone for 5-10 minutes at room temperature. This antibody conjugate has worked well at dilutions of 1:40 - 1:80.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

    References for Anti-Salmonella antibody (FITC) (ab20320)

    ab20320 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab20320.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"