Overview

  • Product name
    Anti-Salmonella antibody (HRP)
    See all Salmonella primary antibodies
  • Description
    Rabbit polyclonal to Salmonella (HRP)
  • Conjugation
    HRP
  • Specificity
    Polyvalent for Salmonella "O" & "H" antigens.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with related Enterobacteriaceae. Not yet tested in other species.
  • Immunogen

    Tissue/ cell preparation (Mixture of S. enteriditis, S. typhimurium and S. heidelburg).

  • General notes

    Covalenty coupled with highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is not present. Estimated molar HRP: IgG substitution is 2-3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

References

This product has been referenced in:
  • Laczka O  et al. Detection of Escherichia coli and Salmonella typhimurium using interdigitated microelectrode capacitive immunosensors: the importance of transducer geometry. Anal Chem 80:7239-47 (2008). Read more (PubMed: 18771278) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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