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The antibody reacts with bacteria in suspension and is therefore useful for detection of Salmonella in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Samonella.
Our Abpromise guarantee covers the use of ab8144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Agglutination||Use at an assay dependent concentration.
Prepare bacteria suspension in a physiological salt solution and place 10 µl on glass slide. Add 10 µl antibody solution, mix well and watch clumping. Incorporate always negative and positive controls.
Dilute in PBS containing 4% BSA + 0.05% Tween. Prepare bacteria suspension in PBS (5x108 bact/ml). Coat ELISA plate with 100 ul/well for 1 hour at 37°C, store overnight at 4°C. Wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 hour at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.
ab8144 has not yet been referenced specifically in any publications.