Anti-Salmonella serogroup E antibody [42-77] (ab8144)

Overview

  • Product nameAnti-Salmonella serogroup E antibody [42-77]
  • Description
    Mouse monoclonal [42-77] to Salmonella serogroup E
  • SpecificityThe antibody reacts with 9 out of 9 tested Salmonella (belonging to serogroup E): The monoclonal reacts with: S. Meleagridis S. Anatum S. Newbrunswick S. Goerlitz S. Canoga S. Minneapolis S. Taksony S. Senftenberg S. Niloese It shows only minor reactivity with a few members of 140 tested non-Salmonella bacteria. No reactivity with other Salmonella serogroups from group A, B, C1, C2, D, K and Y was observed (40 tested).
  • Tested applicationsSuitable for: Agglutination, ELISAmore details
  • General notes


    The antibody reacts with bacteria in suspension and is therefore useful for detection of Salmonella in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Samonella.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • PurityTissue culture supernatant
  • Purification notesAmmonium sulphate precipitation followed by Mono Q-column.
  • Primary antibody notesThe antibody reacts with bacteria in suspension and is therefore useful for detection of Salmonella in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Samonella.
  • ClonalityMonoclonal
  • Clone number42-77
  • IsotypeIgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.

Prepare bacteria suspension in a physiological salt solution and place 10 µl on glass slide. Add 10 µl antibody solution, mix well and watch clumping. Incorporate always negative and positive controls.

ELISA 1/10.

Dilute in PBS containing 4% BSA + 0.05% Tween. Prepare bacteria suspension in PBS (5x108 bact/ml). Coat ELISA plate with 100 ul/well for 1 hour at 37°C, store overnight at 4°C. Wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 hour at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.

References for Anti-Salmonella serogroup E antibody [42-77] (ab8144)

ab8144 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I apologise for the slight delay in response as in this instance it has been necessary to contact the originator of the clone itself to gain this information. The monoclonal reacts with: S. Meleagridis S. Anatum S. New...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"