Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)
Key features and details
- Mouse monoclonal [1E6] to Salmonella typhimurium LPS
- Suitable for: WB, Flow Cyt, Dot blot, ELISA, ICC/IF, Electron Microscopy
- Reacts with: Salmonella typhimurium
- Isotype: IgG1
Overview
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Product name
Anti-Salmonella typhimurium LPS antibody [1E6] -
Description
Mouse monoclonal [1E6] to Salmonella typhimurium LPS -
Host species
Mouse -
Specificity
This antibody is directed against lipopolisaccharides of Salmonella typhimurium -
Tested applications
Suitable for: WB, Flow Cyt, Dot blot, ELISA, ICC/IF, Electron Microscopymore details -
Species reactivity
Reacts with: Salmonella typhimurium -
Immunogen
Full length native protein (purified) corresponding to Salmonella typhimurium Salmonella typhimurium LPS. Lipopolysaccharides of Salmonella typhimurium
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General notes
This antibody is used for the detection of LPS of Salmonella typhimurium.
This product was changed from ascites to tissue culture supernatant on 16th July 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody is used for the detection of LPS of Salmonella typhimurium -
Clonality
Monoclonal -
Clone number
1E6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 17442963
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Dot blot |
Use at an assay dependent concentration.
|
|
ELISA |
Use at an assay dependent concentration.
|
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ICC/IF |
Use at an assay dependent concentration.
|
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Electron Microscopy |
Use at an assay dependent concentration.
|
Notes |
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WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 17442963 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Dot blot
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Electron Microscopy
Use at an assay dependent concentration. |
Target
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Relevance
Salmonellae are Gram-negative , facultatively anaerobic bacteria of the family Enterobacteriaceae, made up of nonspore-forming rods, usually motile with flagella. Salmonella enterica serovar Typhimurium is the among the most common Salmonella serovars causing Salmonellosis food poisoning. S. typhimurium has a less alarming public image than S.typhi (which causes typhoid), but is a bigger health problem worldwide. Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors. -
Alternative names
- S. typhimurium antibody
- Salmonella typhimurium antibody
- SALMONELLA TYPHIMURIUM LPS FACTOR 4 antibody
Images
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Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.
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All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution
Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344
Lysates/proteins at 100000000 cells per lane.
Secondary
All lanes : AP conjugated goat anti-mouse polyclonal at 1/4000 dilution
Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab8274 has been referenced in 26 publications.
- Micoli F et al. Methods for Assessment of OMV/GMMA Quality and Stability. Methods Mol Biol 2414:227-279 (2022). PubMed: 34784041
- Jiang L et al. A bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants. J Extracell Vesicles 11:e12192 (2022). PubMed: 35289114
- Pividori MI Immunomagnetic Separation of Salmonella with Tailored Magnetic Micro- and Nanocarriers. Methods Mol Biol 2182:51-65 (2021). PubMed: 32894487
- Goodwin JM et al. GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis. Sci Adv 7:eabj2485 (2021). PubMed: 34597140
- Ssemakalu CC et al. Solar inactivated Salmonella Typhimurium induces an immune response in BALB/c mice. Heliyon 7:e05903 (2021). PubMed: 33553721