Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)

Overview

  • Product nameAnti-Salmonella typhimurium LPS antibody [1E6]
    See all Salmonella typhimurium LPS primary antibodies
  • Description
    Mouse monoclonal [1E6] to Salmonella typhimurium LPS
  • SpecificityThis antibody is directed against lipopolisaccharides of Salmonella typhimurium
  • Tested applicationsSuitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IF, IHC-Frmore details
  • Immunogen

    Lipopolysaccharides of Salmonella typhimurium

  • General notes


    This antibody is used for the detection of LPS of Salmonella typhimurium

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThis antibody is used for the detection of LPS of Salmonella typhimurium
  • ClonalityMonoclonal
  • Clone number1E6
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 17442963ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceSalmonellae are Gram-negative , facultatively anaerobic bacteria of the family Enterobacteriaceae, made up of nonspore-forming rods, usually motile with flagella. Salmonella enterica serovar Typhimurium is the among the most common Salmonella serovars causing Salmonellosis food poisoning. S. typhimurium has a less alarming public image than S.typhi (which causes typhoid), but is a bigger health problem worldwide. Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.
  • Alternative names
    • S. typhimurium antibody
    • Salmonella typhimurium antibody
    • SALMONELLA TYPHIMURIUM LPS FACTOR 4 antibody

Anti-Salmonella typhimurium LPS antibody [1E6] images

  • Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.

    See Abreview

  • All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution

    Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
    Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
    Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
    Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344

    Lysates/proteins at 100000000 cells per lane.

    Secondary
    AP conjugated goat anti-mouse polyclonal at 1/4000 dilution

    Image courtesy of Aaron White by Abreview.

    Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.

    See Abreview

References for Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)

This product has been referenced in:
  • Zhang K  et al. Age-dependent enterocyte invasion and microcolony formation by Salmonella. PLoS Pathog 10:e1004385 (2014). IHC-P . Read more (PubMed: 25210785) »
  • Eulalio A  et al. A Candidate Approach Implicates the Secreted Salmonella Effector Protein SpvB in P-Body Disassembly. PLoS One 6:e17296 (2011). ICC/IF . Read more (PubMed: 21390246) »

See all 3 Publications for this product

Product Wall


Unfortunately, we do not carry the biotinylated forms of these 2 antibodies, but we do carry biotinylation kits (e.g. http://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html - other sizes exist as well) if you li...

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We have 5 antibodies to Salmonella typhimurium:

ab69238 - was raised against the whole bacteria thus we do not know where the antibody would bind.

ab8274 and ab65922: both were raised against LPS of the bacterium which I suppose is lo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Salmonella Typhimurium Cell lysate - other (Whole cell polysaccharides)
Loading amount 1e+008 cells
Specification Whole cell polysaccharides
Treatment Proteinase K (0.5mg/mL) for 1 h at 65C
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Username

Aaron White

Verified customer

Submitted Jan 27 2012

Thank you for contacting Abcam.

I have no added both Salmonella Typhimurium and Salmonella Enteritidis to our list of species and so you should now be able to submit anAbreview for this antibody.

If there is anything else I can help...

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DISCOUNT CODE: ****** Expiration date:****** I am very pleased to hear you would like to accept our offer and test ab8274 in western blot. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Salmonella paratyphi Tissue sections (Salmonella ultrathin cryosections)
Specification Salmonella ultrathin cryosections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted May 29 2008

Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich imm...

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Both monoclonal antibodies recognize heat-killed Salmonella typhimurium. We have used the monoclonal antibodies (Mab) in ELISA according to the following scheme: antigen coating - Mab - Anti-mouse-IgG (conjugated), not in sandwich ELISA. The antibo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"