Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human SAM68.
(Peptide available as ab27829.)
Our Abpromise guarantee covers the use of ab26803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | Use a concentration of 1 µg/ml. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa). | |
IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Although the calculated molecular weight of SAM68 is 49 kDa, it has been shown to migrate at 68 kDa (see Molecular Biology of the Cell 14(1):274). ab26803 detects a band that is 68 kDa.
ICC/IF image of ab26803 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab26803, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells.
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