Overview

  • Product name
  • Description
    Rabbit polyclonal to SAM68
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of Human SAM68 (NP_006550.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
    Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
  • Tissue specificity
    Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
  • Sequence similarities
    Belongs to the KHDRBS family.
    Contains 1 KH domain.
  • Developmental stage
    Isoform 3 is only expressed in growth-arrested cells.
  • Domain
    The KH domain is required for binding to RNA.
    The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions.
  • Post-translational
    modifications
    Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
    Acetylated. Positively correlates with ability to bind RNA.
    Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
    Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Nucleus. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ34027 antibody
    • GAP associated tyrosine phosphoprotein p62 antibody
    • GAP-associated tyrosine phosphoprotein p62 antibody
    • KH domain containing RNA binding signal transduction associated 1 antibody
    • KH domain-containing antibody
    • KHDR1_HUMAN antibody
    • Khdrbs1 antibody
    • p21 Ras GTPase activating protein associated p62 antibody
    • p21 Ras GTPase-activating protein-associated p62 antibody
    • p62 antibody
    • p68 antibody
    • RNA-binding antibody
    • Sam68 antibody
    • signal transduction-associated protein 1 antibody
    • Src associated in mitosis 68 kDa protein antibody
    • Src-associated in mitosis 68 kDa protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SAM68 with ab86239 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-SAM68 antibody (ab86239) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 seconds
  • Detection of SAM68 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86239 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.

References

ab86239 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
hnRNP C (sc-15386) bound protein A dynabeads 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads
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Submitted Jun 02 2011

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