Recombinant
Anti-SAM68 antibody [EPR3231] (ab76471)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-SAM68 antibody [EPR3231]
See all SAM68 primary antibodies -
DescriptionRabbit monoclonal [EPR3231] to SAM68
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
A synthetic peptide corresponding to residues on the N terminal of human SAM68
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Positive control
- HeLa and Jurkat cell lysates and human brain tissue and Hela cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3231
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab76471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/20000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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FunctionRecruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase. -
Tissue specificityUbiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
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Sequence similaritiesBelongs to the KHDRBS family.
Contains 1 KH domain. -
Developmental stageIsoform 3 is only expressed in growth-arrested cells.
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DomainThe KH domain is required for binding to RNA.
The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions. -
Post-translational
modificationsTyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
Acetylated. Positively correlates with ability to bind RNA.
Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine. -
Cellular localizationNucleus. Membrane.
- Information by UniProt
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Database links
- Entrez Gene: 10657 Human
- Entrez Gene: 20218 Mouse
- Entrez Gene: 117268 Rat
- Omim: 602489 Human
- SwissProt: Q07666 Human
- SwissProt: Q3U8T3 Mouse
- SwissProt: Q60749 Mouse
- SwissProt: Q91V33 Rat
see all -
Alternative names
- FLJ34027 antibody
- GAP associated tyrosine phosphoprotein p62 antibody
- GAP-associated tyrosine phosphoprotein p62 antibody
see all
Images
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![Western blot - SAM68 antibody [EPR3231] (ab76471)](http://a.abcam.com/ps/datasheet/Images/76/ab76471/ab76471_1.jpg)
Western blot - SAM68 antibody [EPR3231] (ab76471)All lanes : Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 68 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAM68 antibody [EPR3231] (ab76471)](http://a.abcam.com/ps/datasheet/Images/76/ab76471/ab76471_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAM68 antibody [EPR3231] (ab76471)ab76471 at 1/100 dilution staining SAM68 in human brain tissue, using a HRP/AP polymerized secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - SAM68 antibody [EPR3231] (ab76471)](http://a.abcam.com/ps/datasheet/Images/76/ab76471/ab76471_3.jpg)
Immunocytochemistry/ Immunofluorescence - SAM68 antibody [EPR3231] (ab76471)Immunofluorescent staining of HeLa cells using ab76471 at a 1/100 dilution.
Protocols
References
This product has been referenced in:
- Rajanala K & Nandicoori VK Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. PLoS One 7:e29921 (2012). WB ; Human . Read more (PubMed: 22253824) »
- Cardnell RJ & Mikkelsen RB Nitric oxide synthase inhibition enhances the antitumor effect of radiation in the treatment of squamous carcinoma xenografts. PLoS One 6:e20147 (2011). IHC . Read more (PubMed: 21647438) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"