Anti-SAM68 antibody [EPR3231] (ab76471)


  • Product name
    Anti-SAM68 antibody [EPR3231]
    See all SAM68 primary antibodies
  • Description
    Rabbit monoclonal [EPR3231] to SAM68
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminal of human SAM68

  • Positive control
    • HeLa and Jurkat cell lysates and human brain tissue and Hela cells
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab76471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
      Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
    • Tissue specificity
      Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
    • Sequence similarities
      Belongs to the KHDRBS family.
      Contains 1 KH domain.
    • Developmental stage
      Isoform 3 is only expressed in growth-arrested cells.
    • Domain
      The KH domain is required for binding to RNA.
      The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions.
    • Post-translational
      Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
      Acetylated. Positively correlates with ability to bind RNA.
      Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
      Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization
      Nucleus. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ34027 antibody
      • GAP associated tyrosine phosphoprotein p62 antibody
      • GAP-associated tyrosine phosphoprotein p62 antibody
      • KH domain containing RNA binding signal transduction associated 1 antibody
      • KH domain-containing antibody
      • KHDR1_HUMAN antibody
      • Khdrbs1 antibody
      • p21 Ras GTPase activating protein associated p62 antibody
      • p21 Ras GTPase-activating protein-associated p62 antibody
      • p62 antibody
      • p68 antibody
      • RNA-binding antibody
      • Sam68 antibody
      • signal transduction-associated protein 1 antibody
      • Src associated in mitosis 68 kDa protein antibody
      • Src-associated in mitosis 68 kDa protein antibody
      see all


    • All lanes : Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 49 kDa
      Observed band size: 68 kDa (why is the actual band size different from the predicted?)

    • ab76471 at 1/100 dilution staining SAM68 in human brain tissue, using a HRP/AP polymerized secondary antibody.
    • Immunofluorescent staining of HeLa cells using ab76471 at a 1/100 dilution.


    This product has been referenced in:
    • Rajanala K & Nandicoori VK Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. PLoS One 7:e29921 (2012). WB ; Human . Read more (PubMed: 22253824) »
    • Cardnell RJ & Mikkelsen RB Nitric oxide synthase inhibition enhances the antitumor effect of radiation in the treatment of squamous carcinoma xenografts. PLoS One 6:e20147 (2011). IHC . Read more (PubMed: 21647438) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (Human Fibroblast)
    Loading amount
    20 µg
    Human Fibroblast
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Jan 09 2013


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