Anti-SAM68 antibody [EPR3231] (ab76471)

Overview

• Product name
  Anti-SAM68 antibody [EPR3231]
  See all SAM68 primary antibodies
• Description
  Rabbit monoclonal [EPR3231] to SAM68
• Tested applications
  Suitable for: WB, IHC-P, ICC/IFmore details
  Unsuitable for: Flow Cyt or IP
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues on the N terminal of human SAM68

• Positive control
  • HeLa and Jurkat cell lysates and human brain tissue and Hela cells
• General notes

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purity
  Tissue culture supernatant
• Clonality
  Monoclonal
• Clone number
  EPR3231
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Adapters
  • Transmembrane

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Associated products

• Positive Controls
  • HeLa nuclear extract lysate (ab14655)
  • Jurkat nuclear extract lysate (ab14844)

Applications

Target

• Function
  Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.
  Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.
• Tissue specificity
  Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.
• Sequence similarities
  Belongs to the KHDRBS family.
  Contains 1 KH domain.
• Developmental stage
  Isoform 3 is only expressed in growth-arrested cells.
• Domain
  The KH domain is required for binding to RNA.
  The Pro-rich domains are flanked by Arg/Gly-rich motifs which can be asymmetric dimethylated on arginine residues to give the DMA/Gly-rich regions. Selective methylation on these motifs can modulate protein-protein interactions.
• Post-translational
  modifications
  Tyrosine phosphorylated by several non-receptor tyrosine kinases, for example LCK, FYN and JAK3. Negatively correlates with ability to bind RNA but required for many interactions with proteins.
  Acetylated. Positively correlates with ability to bind RNA.
  Arginine methylation is required for nuclear localization. Also can affect interaction with other proteins. Inhibits interaction with Src-like SH3 domains, but not interaction with WW domains of WBP4/FBP21 AND FNBP4/FBP30.
  Arg-291, Arg-331 and Arg-346 are found to be also dimethylated, probably to asymmetric dimethylarginine.
• Cellular localization
  Nucleus. Membrane.

• Target information above from: UniProt accession Q07666 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 10657 Human
  • Entrez Gene: 20218 Mouse
  • Entrez Gene: 117268 Rat
  • Omim: 602489 Human
  • SwissProt: Q07666 Human
  • SwissProt: Q3U8T3 Mouse
  • SwissProt: Q60749 Mouse
  • SwissProt: Q91V33 Rat

  • Unigene: 445893 Human
  • Unigene: 8256 Mouse
  • Unigene: 162107 Rat

  see all
• Alternative names
  • FLJ34027 antibody
  • GAP associated tyrosine phosphoprotein p62 antibody
  • GAP-associated tyrosine phosphoprotein p62 antibody

  • KH domain containing RNA binding signal transduction associated 1 antibody
  • KH domain-containing antibody
  • KHDR1_HUMAN antibody
  • Khdrbs1 antibody
  • p21 Ras GTPase activating protein associated p62 antibody
  • p21 Ras GTPase-activating protein-associated p62 antibody
  • p62 antibody
  • p68 antibody
  • RNA-binding antibody
  • Sam68 antibody
  • signal transduction-associated protein 1 antibody
  • Src associated in mitosis 68 kDa protein antibody
  • Src-associated in mitosis 68 kDa protein antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols