Overview

  • Product nameAnti-SAMD12 antibody
  • Description
    Rabbit polyclonal to SAMD12
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LHCGLNPRGI DHPAHAEGIK LQIEGEGVES QSIKNKNFQK VPDQKGTPKR LQAEAETAKS ATVKLSKPVA LWTQQDVCKW LKKHCPNQYQ IYSESF, corresponding to amino acids 6-101 of Human SAMD12 (UniProt ID:Q8N8I0).

  • Positive control
    • Human stomach tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-SAMD12 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-SAMD12 antibody (ab121831) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
  • ab121831, at a 1/100 dilution, staining SAMD12 in paraffin embedded Human stomach tissue by Immunohistochemistry.

References for Anti-SAMD12 antibody (ab121831)

ab121831 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"