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Rabbit polyclonal to SAMD12
antigen sequence: LHCGLNPRGI DHPAHAEGIK LQIEGEGVES QSIKNKNFQK VPDQKGTPKR LQAEAETAKS ATVKLSKPVA LWTQQDVCKW LKKHCPNQYQ IYSESF, corresponding to amino acids 6-101 of Human SAMD12 (UniProt ID:Q8N8I0).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Contains 1 SAM (sterile alpha motif) domain.
Information by UniProt
- SAM domain-containing protein 12 antibody
- SAM12_HUMAN antibody
- Samd12 antibody
- Sterile alpha motif domain-containing protein 12 antibody
Immunocytochemistry/ Immunofluorescence - Anti-SAMD12 antibody (ab121831)
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-SAMD12 antibody (ab121831)
All lanes : Anti-SAMD12 antibody (ab121831) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (ab121831)
ab121831, at a 1/100 dilution, staining SAMD12 in paraffin embedded Human stomach tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"