Overview

  • Product name
  • Description
    Rabbit polyclonal to SAMD9
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VSQKERRETS KQKQKGKENP DMANPSAMST TAKGSKSLKV ELIEDKIDYT KERQPSIDLT CVSYPFDEFS NPYRYKLDFS LQP, corresponding to amino acids 101-183 of Human SAMD9.

  • Positive control
    • Human thyroid gland tissue; RT4 and U 251 MG cell lysates; A431 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Tissue specificity
    Widely expressed. Very low levels in skeletal muscle. Not detected in fetal brain. Down-regulated in aggressive fibromatosis, as well as in breast and colon cancers.
  • Involvement in disease
    Defects in SAMD9 are the cause of normophosphatemic familial tumoral calcinosis (NFTC) [MIM:610455]. NFTC is an uncommon life-threatening disorder characterized by massive periarticular, and seldom visceral, deposition of calcified tumors.
  • Sequence similarities
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • C7orf5 antibody
    • DRIF1 antibody
    • expressed in aggressive fibromatosis antibody
    • KIAA2004 antibody
    • NFTC antibody
    • OEF1 antibody
    • OEF2 antibody
    • SAM domain-containing protein 9 antibody
    • SAMD9 antibody
    • SAMD9_HUMAN antibody
    • sterile alpha motif domain containing 9 antibody
    • Sterile alpha motif domain-containing protein 9 antibody
    see all

Images

  • ab121663, at 1/30 dilution, staining SAMD9 in paraffin-embedded Human thyroid gland tissue by Immunohistochemistry.
  • All lanes : Anti-SAMD9 antibody (ab121663) at 1/250 dilution

    Lane 1 : RT4 cell lysates
    Lane 2 : U251MG cell lysates
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysates
    Lane 5 : Human tonsil lysates

    Developed using the ECL technique
  • ab121663 at 4 µg/ml staining SAMD9 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

References

ab121663 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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