• Product nameAnti-SAMHD1 antibody [1F9]
    See all SAMHD1 primary antibodies
  • Description
    Mouse monoclonal [1F9] to SAMHD1
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human SAMHD1 protein (NP_056289) produced in HEK293T cell.

  • Positive control
    • Hela cells. This antibody gave a positive result in IHC in the following FFPE tissue: human spleen.



Our Abpromise guarantee covers the use of ab117908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB 1/2000. Predicted molecular weight: 72 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionPutative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Tissue specificityExpressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
  • Involvement in diseaseDefects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similaritiesBelongs to the SAMHD1 family.
    Contains 1 HD domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHBL2 antibody
    • DCIP antibody
    • Dendritic cell derived IFNG induced protein antibody
    • Dendritic cell-derived IFNG-induced protein antibody
    • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
    • dNTPase antibody
    • HD domain containing 1 antibody
    • HDDC1 antibody
    • Mg11 antibody
    • Monocyte protein 5 antibody
    • MOP 5 antibody
    • MOP-5 antibody
    • MOP5 antibody
    • OTTHUMP00000030889 antibody
    • SAM domain and HD domain 1 antibody
    • SAM domain and HD domain containing protein 1 antibody
    • SAM domain and HD domain-containing protein 1 antibody
    • SAMH1_HUMAN antibody
    • Samhd1 antibody
    • SBBI88 antibody
    see all

Anti-SAMHD1 antibody [1F9] images

  • Predicted band size : 72 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SAMHD knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab117908 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa. ab117908 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab117908 and ab181602 (loading control to GAPDH) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (ab117908) (Red), compared to a nonspecific negative control antibody (Blue).
  • All lanes : Anti-SAMHD1 antibody [1F9] (ab117908) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with the pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY SAMHD1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 72 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200606)
  • IHC image of SAMHD1 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117908, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SAMHD1 antibody [1F9] (ab117908)

This product has been referenced in:
  • Schaller T  et al. Nuclear import of SAMHD1 is mediated by a classical karyopherin a/ß1 dependent pathway and confers sensitivity to VpxMAC induced ubiquitination and proteasomal degradation. Retrovirology 11:29 (2014). Read more (PubMed: 24712655) »
  • Dragin L  et al. Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply. Retrovirology 10:30 (2013). WB ; Human . Read more (PubMed: 23497353) »

See all 3 Publications for this product

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Merci beaucoup de nous avoir contacté.

Le laboratoire m'avais pu confirmer le protocole détaille qui avait été utilisé pour faire le marquage cytométrie de flux avec le ab117908. Veuillez le trouverle...

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Thanks for your enquiry.

ab117908 is an unconjugated antibody. A labelled secondary could be used for detection. Alternatively we have fluorophore conjugation kits that can be used to directly label the antibody. Please see the following link...

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