Overview

  • Product nameAnti-SAMHD1 antibody
    See all SAMHD1 primary antibodies
  • Description
    Mouse polyclonal to SAMHD1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length SAMHD1 protein (Human)

  • Positive control
    • SAMHD1 transfected 293T cell lysate and human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23076149

Target

  • FunctionPutative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Tissue specificityExpressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
  • Involvement in diseaseDefects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similaritiesBelongs to the SAMHD1 family.
    Contains 1 HD domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHBL2 antibody
    • DCIP antibody
    • Dendritic cell derived IFNG induced protein antibody
    • Dendritic cell-derived IFNG-induced protein antibody
    • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
    • dNTPase antibody
    • HD domain containing 1 antibody
    • HDDC1 antibody
    • Mg11 antibody
    • Monocyte protein 5 antibody
    • MOP 5 antibody
    • MOP-5 antibody
    • MOP5 antibody
    • OTTHUMP00000030889 antibody
    • SAM domain and HD domain 1 antibody
    • SAM domain and HD domain containing protein 1 antibody
    • SAM domain and HD domain-containing protein 1 antibody
    • SAMH1_HUMAN antibody
    • Samhd1 antibody
    • SBBI88 antibody
    see all

Anti-SAMHD1 antibody images

  • Anti-SAMHD1 antibody (ab67820) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • All lanes : Anti-SAMHD1 antibody (ab67820) at 1/500 dilution

    Lane 1 : SAMHD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • Immunofluorescence analysis of HeLa cells expressing SAMHD1 fused to murine NIH3T3 (3T3) cells, staining SAMHD1 with ab67820. HeLa cells expressing the SAMHD1 were fused to murine NIH3T3 cells with PEG to induce heterokaryon formation. Cells were fixed with 4% formalin, permeabilized with 0.5% Triton X-100 before incubating with primary antibody

References for Anti-SAMHD1 antibody (ab67820)

This product has been referenced in:
  • Taylor HE  et al. The innate immune factor apolipoprotein L1 restricts HIV-1 infection. J Virol 88:592-603 (2014). Human . Read more (PubMed: 24173214) »
  • Clifford R  et al. SAMHD1 is mutated recurrently in chronic lymphocytic leukemia and is involved in response to DNA damage. Blood 123:1021-31 (2014). Human . Read more (PubMed: 24335234) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Testis)
Antigen retrieval step None
Permeabilization Yes - Triton X-100
Specification Testis
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde
Username

一恵 垣内

Verified customer

Submitted Jun 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 25 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. All our customer feedback, including complaints are ...

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