Overview

  • Product nameAnti-SAMHD1 antibody
    See all SAMHD1 primary antibodies
  • Description
    Rabbit polyclonal to SAMHD1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 122 and 421 of Human SAMHD1 (NP_056289).

  • Positive control
    • Paraffin-embedded OVCA. 293T cell lines and Raji whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250.

Target

  • FunctionPutative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Tissue specificityExpressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
  • Involvement in diseaseDefects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similaritiesBelongs to the SAMHD1 family.
    Contains 1 HD domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHBL2 antibody
    • DCIP antibody
    • Dendritic cell derived IFNG induced protein antibody
    • Dendritic cell-derived IFNG-induced protein antibody
    • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
    • dNTPase antibody
    • HD domain containing 1 antibody
    • HDDC1 antibody
    • Mg11 antibody
    • Monocyte protein 5 antibody
    • MOP 5 antibody
    • MOP-5 antibody
    • MOP5 antibody
    • OTTHUMP00000030889 antibody
    • SAM domain and HD domain 1 antibody
    • SAM domain and HD domain containing protein 1 antibody
    • SAM domain and HD domain-containing protein 1 antibody
    • SAMH1_HUMAN antibody
    • Samhd1 antibody
    • SBBI88 antibody
    see all

Anti-SAMHD1 antibody images

  • Anti-SAMHD1 antibody (ab96768) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 72 kDa
  • ab96768, at 1/100, staining paraffin-embedded OVCA by Immunohistochemistry.

References for Anti-SAMHD1 antibody (ab96768)

This product has been referenced in:
  • Clifford R  et al. SAMHD1 is mutated recurrently in chronic lymphocytic leukemia and is involved in response to DNA damage. Blood 123:1021-31 (2014). Human . Read more (PubMed: 24335234) »
  • Kim B  et al. Tight Interplay Among SAMHD1 Level, Cellular dNTP Levels and HIV-1 Proviral DNA Synthesis Kinetics in Human Primary Monocyte-Derived Macrophages. J Biol Chem : (2012). WB ; Human . Read more (PubMed: 22589553) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis)
Permeabilization Yes - Triton X-100
Specification Testis
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 14 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 25 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"