Recombinant Anti-SAMM50/SAM50 antibody [EPR8717] (ab167430)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8717] to SAMM50/SAM50
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SAMM50/SAM50 antibody [EPR8717]
See all SAMM50/SAM50 primary antibodies -
Description
Rabbit monoclonal [EPR8717] to SAMM50/SAM50 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart lysate; A431, HeLa and HepG2 cell lysates; Human stomach tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8717 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/50000 - 1/200000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/50000 - 1/200000. Predicted molecular weight: 52 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be required for the assembly pathway of mitochondrial outer membrane proteins. -
Sequence similarities
Belongs to the SAM50/omp85 family. -
Domain
Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure. -
Cellular localization
Mitochondrion outer membrane. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 25813 Human
- Entrez Gene: 68653 Mouse
- Entrez Gene: 300111 Rat
- Omim: 612058 Human
- SwissProt: Q9Y512 Human
- SwissProt: Q8BGH2 Mouse
- SwissProt: Q6AXV4 Rat
- Unigene: 505824 Human
see all -
Alternative names
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
see all
Images
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All lanes : Anti-SAMM50/SAM50 antibody [EPR8717] (ab167430) at 1/50000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue, labeling SAMM50/SAM50 with ab167430 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling SAMM50/SAM50 with ab167430 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab167430 has been referenced in 1 publication.
- Kelly FD et al. Toxoplasma gondii MAF1b Binds the Host Cell MIB Complex To Mediate Mitochondrial Association. mSphere 2:N/A (0). PubMed: 28567444