Overview

  • Product nameAnti-SAMSN1 antibody
    See all SAMSN1 primary antibodies
  • Description
    Rabbit polyclonal to SAMSN1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: YVDVISEEEA APKKIKANRR SNSKKSKTLQ EFLERIHLQE YTSTLLLNGY , corresponding to amino acids 218-267 of Human SAMSN1

  • Positive control
    • Human fetal muscle lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • FunctionNegative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation.
  • Tissue specificityDetected in peripheral blood B-cells (at protein level). Detected in spleen, liver and peripheral blood.
  • Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Cellular localizationNucleus. Cytoplasm. Cell projection > ruffle. Shuttles between cytoplasm and nucleus. Colocalizes with the actin cytoskeleton and actin-rich membrane ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • HACS1 antibody
    • Hematopoietic adapter containing SH3 and sterile α motif (SAM) domains 1 antibody
    • Hematopoietic adapter containing SH3 and sterile alpha motif (SAM) domains 1 antibody
    • Hematopoietic adaptor containing SH3 and SAM domains 1 antibody
    • Nash1 antibody
    • Nuclear localization signals SAM and SH3 domain containing 1 antibody
    • SAM and SH3 domain containing 2 antibody
    • SAM domain antibody
    • SAM domain containing protein SAMSN 1 antibody
    • SAM domain SH3 domain and nuclear localisation signals 1 antibody
    • SAM domain SH3 domain and nuclear localization signals 1 antibody
    • SAM domain, SH3 domain and nuclear localization signals protein 1 antibody
    • SAM domain-containing protein SAMSN-1 antibody
    • SAMN1_HUMAN antibody
    • SAMSN 1 antibody
    • Samsn1 antibody
    • SASH2 antibody
    • SH3 domain and nuclear localisation signals 1 antibody
    • SH3 domain and nuclear localization signals protein 1 antibody
    • SH3 SAM adaptor protein antibody
    • SH3-SAM adaptor protein antibody
    • SH3D6B antibody
    • SLy2 antibody
    • Src homology domain 3 (SH3) containing adapter protein SH3 lymphocyte protein 2 antibody
    see all

Anti-SAMSN1 antibody images

  • Anti-SAMSN1 antibody (ab76389) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-SAMSN1 antibody (ab76389)

ab76389 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"