Anti-SAP130 antibody (ab111739)
Key features and details
- Rabbit polyclonal to SAP130
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAP130 antibody
See all SAP130 primary antibodies -
Description
Rabbit polyclonal to SAP130 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken -
Immunogen
Recombinant fragment corresponding to Human SAP130 aa 771-1005.
Database link: Q9H0E3 -
Positive control
- MCF7 whole cell lysate; NT2D1, IMR32 and U87-MG cell lysates; PC14 xenograft; MCF7 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 110 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or Tris-EDTA buffer, pH 8.0).
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 110 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or Tris-EDTA buffer, pH 8.0). |
ICC/IF
1/100 - 1/500. |
Target
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Function
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. -
Tissue specificity
Expressed in various cancer cell ines. -
Sequence similarities
Belongs to the SAP130 family. -
Domain
The N-terminus may interact with a transcriptional coactivator.
The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors. -
Post-translational
modificationsAcetylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 424756 Chicken
- Entrez Gene: 79595 Human
- Entrez Gene: 269003 Mouse
- Omim: 609697 Human
- SwissProt: Q5F3U0 Chicken
- SwissProt: Q9H0E3 Human
- SwissProt: Q8BIH0 Mouse
- Unigene: 32995 Human
see all -
Alternative names
- 130 kDa Sin3-associated polypeptide antibody
- FLJ12761 antibody
- Histone deacetylase complex subunit SAP130 antibody
see all
Images
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Anti-SAP130 antibody (ab111739) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 110 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of SAP130 in paraformaldehyde-fixed MCF7, using ab111739 at a 1/500 dilution. Lower image was costained with Hoechst 33342.
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Immunohistochemical analysis of SAP130 in paraffin embedded PC14 xenograft, using ab111739 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab111739 has been referenced in 4 publications.
- Metselaar PI et al. Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130). Cells 10:N/A (2021). PubMed: 33800128
- Braschi B et al. The risks of using unapproved gene symbols. Am J Hum Genet 108:1813-1816 (2021). PubMed: 34626580
- Liu K et al. Spliceosome-associated protein 130: a novel biomarker for idiopathic pulmonary fibrosis. Ann Transl Med 8:986 (2020). PubMed: 32953786
- Salas-Armenteros I et al. Human THO-Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability. EMBO J 36:3532-3547 (2017). PubMed: 29074626