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Rabbit polyclonal to SAP130
Predicted to work with:
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 771 and 1005 of Human SAP130 (UniProt ID: Q9H0E3).
MCF7 whole cell lysate; NT2D1, IMR32 and U87-MG cell lysates; PC14 xenograft; MCF7 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 99% PBS, 1% BSA, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 110 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or Tris-EDTA buffer, pH 8.0).
1/100 - 1/500.
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Expressed in various cancer cell ines.
Belongs to the SAP130 family.
The N-terminus may interact with a transcriptional coactivator.
The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
Information by UniProt
130 kDa Sin3-associated polypeptide antibody
Histone deacetylase complex subunit SAP130 antibody
Western blot - Anti-SAP130 antibody (ab111739)
Anti-SAP130 antibody (ab111739) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 110 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-SAP130 antibody (ab111739)
Immunofluorescence analysis of SAP130 in paraformaldehyde-fixed MCF7, using ab111739 at a 1/500 dilution. Lower image was costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP130 antibody (ab111739)
Immunohistochemical analysis of SAP130 in paraffin embedded PC14 xenograft, using ab111739 at a 1/500 dilution.
has not yet been referenced specifically in any publications.
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