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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer pH: 7.00 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 99% PBS, 1% BSA, 20% Glycerol
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 110 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or Tris-EDTA buffer, pH 8.0).
1/100 - 1/500.
Function Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Tissue specificity Expressed in various cancer cell ines.
Sequence similarities Belongs to the SAP130 family.
Domain The N-terminus may interact with a transcriptional coactivator. The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
Post-translational modifications Acetylated.
Cellular localization Nucleus.
Information by UniProt
130 kDa Sin3-associated polypeptide antibody
Histone deacetylase complex subunit SAP130 antibody
Anti-SAP130 antibody images
Western blot - SAP130 antibody (ab111739)
Anti-SAP130 antibody (ab111739) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg Predicted band size : 110 kDa
Immunocytochemistry/ Immunofluorescence - SAP130 antibody (ab111739)
Immunofluorescence analysis of SAP130 in paraformaldehyde-fixed MCF7, using ab111739 at a 1/500 dilution. Lower image was costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAP130 antibody (ab111739)
Immunohistochemical analysis of SAP130 in paraffin embedded PC14 xenograft, using ab111739 at a 1/500 dilution.
References for Anti-SAP130 antibody (ab111739)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"