Overview

  • Product nameAnti-SAP130 antibody
    See all SAP130 primary antibodies
  • Description
    Goat polyclonal to SAP130
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide: VSKKLEDIRTRYAF, corresponding to C terminal amino acids 1204-1217 of Human SAP130.

  • Positive control
    • HeLa or Raji Lysate.
  • General notesPrincipal Names – SF3B3; RSE1; SAP130; SF3B130; SF3b130; KIAA0017; splicing factor 3b, subunit 3, 130kDa; spliceosome-associated protein 130; splicing factor 3b, subunit 3, 130 kDa. Official Gene Symbol - SF3B3. GenBank Accession Number – NP_036558. LocusLink ID - 23450 (human); 101943 (mouse). Gene Ontology terms - pre-mRNA splicing factor activity; small nuclear ribonucleoprotein; mRNA processing; mRNA splicing; protein complex assembly; spliceosome complex.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 136 kDa).Can be blocked with Human SAP130 peptide (ab22952).

Target

  • FunctionActs as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
  • Tissue specificityExpressed in various cancer cell ines.
  • Sequence similaritiesBelongs to the SAP130 family.
  • DomainThe N-terminus may interact with a transcriptional coactivator.
    The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
  • Post-translational
    modifications
    Acetylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa Sin3-associated polypeptide antibody
    • FLJ12761 antibody
    • Histone deacetylase complex subunit SAP130 antibody
    • KIAA0017 antibody
    • mSin3A associated protein 130 antibody
    • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
    • RSE1 antibody
    • SAP 130 antibody
    • Sap130 antibody
    • SF3b 130 antibody
    • SF3b130 antibody
    • SF3B3 antibody
    • Sin3-associated polypeptide p130 antibody
    • Sin3A associated protein 130kDa antibody
    • Sin3A associated protein antibody
    • SP130_HUMAN antibody
    • Spliceosome associated protein 130 antibody
    • Splicing factor 3b subunit 3 130kDa antibody
    • Splicing factor 3b subunit 3 antibody
    • STAF 130 antibody
    • STAF130 antibody
    see all

Anti-SAP130 antibody images

  • Anti-SAP130 antibody (ab3608) at 0.1 µg/ml + HeLa cells, nuclear lysate at 30 µg

    Predicted band size : 136 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab3608 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3608, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SAP130 antibody (ab3608)

This product has been referenced in:
  • Zhang Z  et al. Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the survival of motoneuron (SMN) pre-mRNA. J Biol Chem : (2011). WB ; Human . Read more (PubMed: 21220421) »
  • Yoshimoto R  et al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB ; Human . Read more (PubMed: 19103666) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 50 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 25 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"