• Product nameAnti-SAP155 antibody
    See all SAP155 primary antibodies
  • Description
    Rabbit polyclonal to SAP155
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: PKMNARTYMD VMREQHLTKE EREIRQQLAE KAKAGELKVV NGAAASQPPS, corresponding to N terminal amino acids 145-194 of Human SAP155

  • Positive control
    • Jurkat cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab66664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.


  • FunctionSubunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Sequence similaritiesBelongs to the SF3B1 family.
    Contains 11 HEAT repeats.
  • Post-translational
    Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
  • Cellular localizationNucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hsh155 antibody
    • OTTHUMP00000205700 antibody
    • OTTHUMP00000205702 antibody
    • OTTHUMP00000225001 antibody
    • OTTHUMP00000225002 antibody
    • Pre mRNA processing 10 antibody
    • Pre mRNA splicing factor SF3b, 155 kDa subunit antibody
    • Pre-mRNA splicing factor SF3b 155 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 155 kDa subunit antibody
    • PRP10 antibody
    • PRPF10 antibody
    • SAP 155 antibody
    • SAP155 antibody
    • sf3b1 antibody
    • SF3B1_HUMAN antibody
    • SF3b155 antibody
    • Spliceosome associated protein 155 antibody
    • Spliceosome-associated protein 155 antibody
    • Splicing factor 3B subunit 1 antibody
    • Splicing factor 3b, subunit 1, 155kDa antibody
    see all

Anti-SAP155 antibody images

  • All lanes : Anti-SAP155 antibody (ab66664) at 0.3125 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 146 kDa
    Observed band size : 146 kDa
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SAP155 antibody (ab66664)

ab66664 has not yet been referenced specifically in any publications.

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