Overview

  • Product name
  • Description
    Rabbit polyclonal to SAP155
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR, corresponding to a region within N terminal amino acids 1-50 of Human SAP155

  • Positive control
    • fetal thymus cell lysate, human liver tissue, human skin tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Sequence similarities
    Belongs to the SF3B1 family.
    Contains 11 HEAT repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
  • Cellular localization
    Nucleus speckle. During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hsh155 antibody
    • OTTHUMP00000205700 antibody
    • OTTHUMP00000205702 antibody
    • OTTHUMP00000225001 antibody
    • OTTHUMP00000225002 antibody
    • Pre mRNA processing 10 antibody
    • Pre mRNA splicing factor SF3b, 155 kDa subunit antibody
    • Pre-mRNA splicing factor SF3b 155 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 155 kDa subunit antibody
    • PRP10 antibody
    • PRPF10 antibody
    • SAP 155 antibody
    • SAP155 antibody
    • sf3b1 antibody
    • SF3B1_HUMAN antibody
    • SF3b155 antibody
    • Spliceosome associated protein 155 antibody
    • Spliceosome-associated protein 155 antibody
    • Splicing factor 3B subunit 1 antibody
    • Splicing factor 3b, subunit 1, 155kDa antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SAP155 with ab66774 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling SAP155 with ab66774 at 16µg/ml. Arrows indicate positively labelled epidermal cells. Magnification: 400X.

  • Anti-SAP155 antibody (ab66774) at 1/1.25 dilution + fetal thymus cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 146 kDa
    Observed band size : 146 kDa
    Additional bands at : >168 kDa. We are unsure as to the identity of these extra bands.gel concentration: 8%
  • ICC/IF image of ab66774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66774, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Soboleva TA  et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . Read more (PubMed: 28234895) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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