Overview

  • Product nameAnti-SAP18 antibody
    See all SAP18 primary antibodies
  • Description
    Rabbit polyclonal to SAP18
  • Tested applicationsSuitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SAP18.

    (Peptide available as ab30465.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Nuclear Lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab31748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
ICC/IF
IHC-P
WB
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/650. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
    IP: Use at a concentration of 1.6 µg/ml (PMID 19503603).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). Detection of the band is blocked by addition of the immunizing peptide ab30465.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the SAP18 family.
    • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 18 kDa Sin3-associated polypeptide antibody
      • 2HOR0202 antibody
      • Cell growth-inhibiting gene 38 protein antibody
      • Histone deacetylase complex subunit SAP18 antibody
      • SAP18 antibody
      • SAP18_HUMAN antibody
      • Sin3-associated polypeptide p18 antibody
      see all

    Anti-SAP18 antibody images

    • All lanes : Anti-SAP18 antibody (ab31748) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human SAP18 peptide (ab30465) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 18 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab31748 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31748, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • Image courtesy of Human Protein Atlas
      ab31748 stainning SAP18 in paraffin-embedded human cervix, showing a distinct nuclear staining of the surface epithelial (squamous) cells. Tissue sections (4µm) were incubated with ab31748 (1/650 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
      ab31748 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    References for Anti-SAP18 antibody (ab31748)

    This product has been referenced in:
    • Sorin M  et al. Recruitment of a SAP18-HDAC1 complex into HIV-1 virions and its requirement for viral replication. PLoS Pathog 5:e1000463 (2009). WB, IP . Read more (PubMed: 19503603) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry. This antibody is indeed expected to cross react to the following species: Mouse, Rat, Cow, Xenopus laevis and Zebrafish. But this has not yet been experimentally tested. Unfortunately, this antibody's immunogen only sh...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 50 µg
    Specification HeLa
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted May 01 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"