Overview

  • Product nameAnti-SAP30 antibody
    See all SAP30 primary antibodies
  • Description
    Rabbit polyclonal to SAP30
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Dog, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 170-220 of Human SAP30 (NP_003855.1).

  • Positive control
    • Hela, Jurkat, NIH 3T3 and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab125187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionInvolved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
  • Tissue specificityExpressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
  • Sequence similaritiesBelongs to the SAP30 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa Sin3-associated polypeptide antibody
    • Histone deacetylase complex subunit SAP 30 antibody
    • Histone deacetylase complex subunit SAP30 antibody
    • MSin3A associated polypeptide p30 antibody
    • SAP 30 antibody
    • Sap30 antibody
    • SAP30_HUMAN antibody
    • Sin3 associated polypeptide 30 kDa antibody
    • Sin3 associated polypeptide 30kD antibody
    • Sin3 associated polypeptide antibody
    • Sin3 corepressor complex subunit SAP 30 antibody
    • Sin3 corepressor complex subunit SAP30 antibody
    • Sin3-associated polypeptide p30 antibody
    • Sin3A associated protein 30kDa antibody
    • Sin3A associated protein antibody
    see all

Anti-SAP30 antibody images

  • All lanes : Anti-SAP30 antibody (ab125187) at 0.4 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 23 kDa


    Exposure time : 30 seconds
  • ab125187, at 6 µg/mg lysate, detecting SAP30 in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125187 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 10 seconds.

References for Anti-SAP30 antibody (ab125187)

ab125187 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebra finch Tissue lysate - whole (cultured embryonic fibroblasts, whole embryo head)
Gel Running Conditions Non-reduced Denaturing
Loading amount 100000 cells
Specification cultured embryonic fibroblasts, whole embryo head
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"