Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number4G8
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionSubunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
  • Sequence similaritiesBelongs to the SF3A2 family.
    Contains 1 matrin-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • pre-mRNA splicing factor SF3A, subunit 2 antibody
    • PRP11 antibody
    • PRPF11 antibody
    • SAP 62 antibody
    • Sf3a2 antibody
    • SF3A2_HUMAN antibody
    • SF3a66 antibody
    • spliceosome associated protein 62 antibody
    • Spliceosome-associated protein 62 antibody
    • Splicing factor 3A subunit 2 antibody
    • splicing factor 3a, subunit 2, 66kDa antibody
    see all

Anti-SAP62 antibody [4G8] images

  • ICC/IF image of ab77800 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal gost serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77800, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • All lanes : Anti-SAP62 antibody [4G8] (ab77800) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
    The 60 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SAP62.
  • IHC image of ab77800 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77800, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Overlay histogram showing HeLa cells stained with ab77800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77800, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-SAP62 antibody [4G8] (ab77800)

This product has been referenced in:
  • Sasaki-Osugi K  et al. Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA. J Biol Chem 288:33361-75 (2013). Read more (PubMed: 24078636) »
  • Hong W  et al. Association of U2 snRNP with the spliceosomal complex E. Nucleic Acids Res 25:354-61 (1997). Read more (PubMed: 9016565) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab77800.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"