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Rabbit polyclonal to SAP62
Predicted to work with:
Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant protein fragment, corresponding to a region within amino acids 2-240 of Human SAP62 (UniProt ID: Q15428).
HeLa cell lysate; HeLa cells; Human J5 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
1/100 - 1/1000.
Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Belongs to the SF3A2 family.
Contains 1 matrin-type zinc finger.
Information by UniProt
pre-mRNA splicing factor SF3A, subunit 2 antibody
Western blot - Anti-SAP62 antibody (ab155652)
Anti-SAP62 antibody (ab155652) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 49 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP62 antibody (ab155652)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human J5 xenograft tissue, labeling SAP62 using ab155652 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SAP62 antibody (ab155652)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling SAP62 using ab155652 at a 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"