Overview

  • Product name
  • Description
    Rabbit polyclonal to SAP62
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 2-240 of Human SAP62 (UniProt ID: Q15428).

  • Positive control
    • HeLa cell lysate; HeLa cells; Human J5 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-SAP62 antibody (ab155652) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human J5 xenograft tissue, labeling SAP62 using ab155652 at a 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling SAP62 using ab155652 at a 1/500 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155652 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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