Overview

  • Product nameAnti-SAPAP3 antibody
    See all SAPAP3 primary antibodies
  • Description
    Rabbit polyclonal to SAPAP3
  • Tested applicationsSuitable for: WB, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid peptide derived from near the carboxy terminus of human SAPAP3.

  • Positive control
    • Rat Brain Tissue Lysate, human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab67224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ICC/IF Use a concentration of 20 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceSAPAP3, (DLGAP3) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP3 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). Both SAPAP3 protein and mRNA are targeted to dendrites, whereas SAPAP1, -2, and -4 mRNAs are detected mainly in cell bodies. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. At least two isoforms are known to exist.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • DLGAP3 antibody
    • DAP3 antibody
    • Discs, large (Drosophila) homolog associated protein 3 antibody
    • Disks large associated protein 3 antibody
    • PSD 95/SAP90 binding protein 3 antibody
    • SAP90/PSD 95 associated protein 3 antibody
    • SAPAP 3 antibody
    see all

Anti-SAPAP3 antibody images

  • Immunofluorescence of SAPAP3 in Human brain tissue with ab67224 at 20 ug/mL.

  • Lane 1 : Anti-SAPAP3 antibody (ab67224) at 1 µg/ml
    Lane 2 : Anti-SAPAP3 antibody (ab67224) at 2 µg/ml

    Lane 1 : rat brain tissue lysate
    Lane 2 : rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 106 kDa
    Observed band size : 106 kDa
  • ab67224 at 2.5µg/ml staining of SAPAP3 in human brain tissue  using IHC-P.

References for Anti-SAPAP3 antibody (ab67224)

ab67224 has not yet been referenced specifically in any publications.

Product Wall

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Indeed, you do not need to mention the code for the order of the antibody to test. So everything is perfect. You only need to mention the code when submitting the feedback via an Abr...

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I am very pleased to hear you would like to accept our offer and testab67224 in IP and rabbit. This code will give you: 1 free primary antibody per discount code before the expiration date. To redeem this offer, please submit an Abreview fo...

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I am happy to hear that you are interested in this offer. I am however sorry, that I can not provide you with two vials for testing this antibody in IP. If however you have any other application or species you could...

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Thank you very much for your interest in our anti SAPAP3 antibody ab67224.

We do not know whether the ab67224 is suitable for immunoprecipitation (IP), as we have not tested this experimentally. The chances are there, as the antibody does rec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"