Overview

  • Product nameAnti-SAPS2 antibody
    See all SAPS2 primary antibodies
  • Description
    Rabbit polyclonal to SAPS2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 875 and the C-terminus (residue 932) of human SAPS2, (NP_055493.2).

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab72032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 105 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceSAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C). Protein phosphatase regulatory subunits, such as SAPS2, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. It is ubiquitously expressed with strongest expression in the testis followed by liver, heart, kidney, brain and placenta. There are two named isoforms.
  • Cellular localizationCytoplasm
  • Database links
  • Alternative names
    • SAPS 2 antibody
    • dJ579N16.1 antibody
    • KIAA0685 antibody
    • PP6R2 antibody
    • SAP190 antibody
    • SAPS domain family, member 2 antibody
    see all

Anti-SAPS2 antibody images

  • All lanes : Anti-SAPS2 antibody (ab72032) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 105 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of SAPS2.
    Lane 1: ab72032 at 3µg/mg whole cell lysate for IP,
    followed by ab72032 at 1µg/ml for WB.
    Lane 2: Control IgG.

    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.

References for Anti-SAPS2 antibody (ab72032)

ab72032 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"