Anti-SAPS3 antibody (ab72034)
Key features and details
- Rabbit polyclonal to SAPS3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAPS3 antibody -
Description
Rabbit polyclonal to SAPS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide from a region between residue 743 and the C-terminus (residue 793) of human SAPS3,( NP_060782.2).
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Positive control
- Whole cell lysate from Hela cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance. -
Tissue specificity
Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes. -
Sequence similarities
Belongs to the SAPS family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55291 Human
- Omim: 610879 Human
- SwissProt: Q5H9R7 Human
- Unigene: 503022 Human
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Alternative names
- C11orf23 antibody
- Chromosome 11 open reading frame 23 antibody
- KIAA1558 antibody
see all
Images
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All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 98 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunoprecipitation/ Western Blot of SAPS3.
Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72034 has been referenced in 1 publication.
- Gallegos LL et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161