Overview

  • Product name
    Anti-SAPS3 antibody
  • Description
    Rabbit polyclonal to SAPS3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide from a region between residue 743 and the C-terminus (residue 793) of human SAPS3,( NP_060782.2).

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance.
  • Tissue specificity
    Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes.
  • Sequence similarities
    Belongs to the SAPS family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf23 antibody
    • Chromosome 11 open reading frame 23 antibody
    • KIAA1558 antibody
    • PP6R3 antibody
    • PP6R3_HUMAN antibody
    • PPP6R3 antibody
    • Protein phosphatase 6 regulatory subunit 3 antibody
    • SAP190 antibody
    • SAPL antibody
    • SAPLa antibody
    • SAPS domain family member 3 antibody
    • SAPS domain family, member 3 antibody
    • Serine/threonine-protein phosphatase 6 regulatory subunit 3 antibody
    • Sporulation induced transcript 4 associated protein SAPL antibody
    • Sporulation-induced transcript 4-associated protein SAPL antibody
    see all

Images

  • All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 98 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of SAPS3.
    Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
    Lane 2: Control IgG.

    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:
  • Gallegos LL  et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). Read more (PubMed: 27255161) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72034.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up