Overview

  • Product name
    Anti-SAR1 antibody [3G5]
    See all SAR1 primary antibodies
  • Description
    Mouse monoclonal [3G5] to SAR1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein (Human) (AAH03658) with a 26 kDa tag.

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
  • Sequence similarities
    Belongs to the small GTPase superfamily. SAR1 family.
  • Cellular localization
    Endoplasmic reticulum. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COPII associated small GTPase antibody
    • COPII-associated small GTPase antibody
    • GTP binding protein SAR1a antibody
    • GTP-binding protein SAR1a antibody
    • Masra 2 antibody
    • Masra2 antibody
    • SAR 1 antibody
    • SAR1 gene homolog A antibody
    • SAR1 homolog A (S. cerevisiae) antibody
    • SAR1A antibody
    • SAR1a gene homolog (S cerevisiae) 1 antibody
    • SAR1a gene homolog 1 (S cerevisiae) antibody
    • SAR1a gene homolog 1 antibody
    • SAR1A_HUMAN antibody
    • SARA 1 antibody
    • Sara antibody
    • SARA1 antibody
    see all

Anti-SAR1 antibody [3G5] images

  • Anti-SAR1 antibody [3G5] (ab77029) at 5 µg/ml + recombinant full length human SAR1 protein with a tag (immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Anti-SAR1 antibody [3G5] (ab77029) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab77029 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77029, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab77029 staining SAR1 in Panc-1 cell line from Human pancreas carcinoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formaldehyde and blocked with 1% Donkey Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/75 in PBST + 1% Serum) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-SAR1 antibody [3G5] (ab77029)

ab77029 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jan 19 2016

Application
Western blot
Sample
Rat Cell lysate - other (Golgi fraction)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
15 µg
Specification
Golgi fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 27 2015

Application
Western blot
Loading amount
18 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (Panc-1)
Specification
Panc-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Specification
Panc-1
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Dec 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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