Overview

  • Product name
  • Description
    Goat polyclonal to SAR1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Physarum polycephalum
  • Immunogen

    Synthetic peptide:

    C-HSRLVESKVELNA

    , corresponding to internal sequence amino acids 105-117 of Human SAR1 (NP_064535.1).

  • Positive control
    • NIH3T3 lysate; Human small intestine and skin tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab111814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 22 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
  • Sequence similarities
    Belongs to the small GTPase superfamily. SAR1 family.
  • Cellular localization
    Endoplasmic reticulum. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COPII associated small GTPase antibody
    • COPII-associated small GTPase antibody
    • GTP binding protein SAR1a antibody
    • GTP-binding protein SAR1a antibody
    • Masra 2 antibody
    • Masra2 antibody
    • SAR 1 antibody
    • SAR1 gene homolog A antibody
    • SAR1 homolog A (S. cerevisiae) antibody
    • SAR1A antibody
    • SAR1a gene homolog (S cerevisiae) 1 antibody
    • SAR1a gene homolog 1 (S cerevisiae) antibody
    • SAR1a gene homolog 1 antibody
    • SAR1A_HUMAN antibody
    • SARA 1 antibody
    • Sara antibody
    • SARA1 antibody
    see all

Images

  • Anti-SAR1 antibody (ab111814) at 0.1 µg/ml + NIH3T3 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 22 kDa
  • ab111814 at 3.75 µg/ml staining SAR1 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry.
  • ab111814 at 3.75 µg/ml staining SAR1 in Formalin-fixed, Paraffin-embedded Human Skin tissue by Immunohistochemistry.

References

ab111814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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