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Rabbit polyclonal to SAR1
Predicted to work with:
Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish
Recombinant fragment, corresponding to a region within N terminal amino acids 6-180 of Human SAR1 (Q9NR31).
293T and A431 whole cell lysates; A431 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Immunogen affinity purified
ab125871 is purified by antigen-affinity chromatography.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
1/100 - 1/500.
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Belongs to the small GTPase superfamily. SAR1 family.
Endoplasmic reticulum. Golgi apparatus.
Information by UniProt
COPII associated small GTPase antibody
COPII-associated small GTPase antibody
GTP binding protein SAR1a antibody
Western blot - Anti-SAR1 antibody (ab125871)
All lanes : Anti-SAR1 antibody (ab125871) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 22 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-SAR1 antibody (ab125871)
ab125871, at 1/500, staining SAR1 in methanol-fixed A431 cells by Immunofluorescence. Lower image is co-stained with Hoechst 33342.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"