Overview

  • Product nameAnti-SARA antibody [EPR5020]
    See all SARA primary antibodies
  • Description
    Rabbit monoclonal [EPR5020] to SARA
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the N-terminus of Human SARA.

  • Positive control
    • Huan heart, A673, HT-1080, BxPC-3, Jutkat, HeLa, and Human fetal brain lysates, Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionEarly endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
    • Tissue specificityUbiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
    • Sequence similaritiesContains 1 FYVE-type zinc finger.
    • DomainThe SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3.
      The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
    • Cellular localizationCytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CG15667 antibody
      • hSARA antibody
      • LD33044p antibody
      • Madh-interacting protein antibody
      • MADHIP antibody
      • Mothers against decapentaplegic homolog-interacting protein antibody
      • Novel serine protease antibody
      • NSP antibody
      • Receptor activation anchor antibody
      • SAR1 antibody
      • SARA antibody
      • SARA1 antibody
      • Smad anchor for receptor activation antibody
      • SMADIP antibody
      • ZFYV9_HUMAN antibody
      • ZFYVE9 antibody
      • Zinc finger FYVE domain-containing protein 9 antibody
      see all

    Anti-SARA antibody [EPR5020] images

    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HT-1080 cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 156 kDa
    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 156 kDa
    • Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-SARA antibody [EPR5020] (ab124875)

    ab124875 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124875.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"