The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
1/10 - 1/100.
1/100 - 1/250.
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for ICC.
FunctionEarly endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
Tissue specificityUbiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
DomainThe SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3. The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
Cellular localizationCytoplasm. Early endosome membrane.