Overview

  • Product name
    Anti-SARA antibody [EPR5020]
    See all SARA primary antibodies
  • Description
    Rabbit monoclonal [EPR5020] to SARA
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the N-terminus of Human SARA.

  • Positive control
    • Huan heart, A673, HT-1080, BxPC-3, Jutkat, HeLa, and Human fetal brain lysates, Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 180 kDa (predicted molecular weight: 156 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.
    • Tissue specificity
      Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.
    • Sequence similarities
      Contains 1 FYVE-type zinc finger.
    • Domain
      The SMAD binding domain (SBD) interacts with the MH2 domains of SMAD2 or SMAD3.
      The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
    • Cellular localization
      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CG15667 antibody
      • hSARA antibody
      • LD33044p antibody
      • Madh-interacting protein antibody
      • MADHIP antibody
      • Mothers against decapentaplegic homolog-interacting protein antibody
      • Novel serine protease antibody
      • NSP antibody
      • Receptor activation anchor antibody
      • SAR1 antibody
      • SARA antibody
      • SARA1 antibody
      • Smad anchor for receptor activation antibody
      • SMADIP antibody
      • ZFYV9_HUMAN antibody
      • ZFYVE9 antibody
      • Zinc finger FYVE domain-containing protein 9 antibody
      see all

    Images

    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HT-1080 cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 156 kDa
    • All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 156 kDa
    • Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Jing C  et al. MicroRNA-17/20a impedes migration and invasion via TGF-ß/ITGB6 pathway in esophageal squamous cell carcinoma. Am J Cancer Res 6:1549-62 (2016). Read more (PubMed: 27508097) »
    • Correia AC  et al. FGF2 inhibits endothelial-mesenchymal transition through microRNA-20a-mediated repression of canonical TGF-ß signaling. J Cell Sci 129:569-79 (2016). Read more (PubMed: 26729221) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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