Overview

  • Product name
    Anti-Sarcalumenin antibody [XIIC4]
    See all Sarcalumenin primary antibodies
  • Description
    Mouse monoclonal [XIIC4] to Sarcalumenin
  • Specificity
    This antibody specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant.
  • Tested applications
    Suitable for: ICC/IF, WB, Inhibition Assay, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Guinea pig, Dog, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit Sarcalumenin. Purified from rabbit sarcoplasmic reticulum membrane vesicles.

  • Positive control
    • Rabbit skeletal muscle extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner.The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.
  • Clonality
    Monoclonal
  • Clone number
    XIIC4
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.

Immunofluorescence staining of sarcalumenin and its splice variant in rabbit skeletal muscle results in staining that co-localizes with calcium ATPase in the longitudinal sarcoplasmic reticulum (SR) and the terminal cisternae.

WB 1/500.

By Western blot, this antibody detects a 53 kDa and a 160 kDa protein representing the glycoprotein and sarcalumenin, respectively, in rabbit skeletal muscle extracts.

Inhibition Assay Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr 1/100.

Target

References

This product has been referenced in:
  • Kutchai H & Campbell KP Calcium transport by sarcoplasmic reticulum of skeletal muscle is inhibited by antibodies against the 53-kilodalton glycoprotein of the sarcoplasmic reticulum membrane. Biochemistry 28:4830-9 (1989). Read more (PubMed: 2527558) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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