Anti-Sarcomeric Alpha Actinin antibody (ab113086)

Overview

  • Product name
    Anti-Sarcomeric Alpha Actinin antibody
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit polyclonal to Sarcomeric Alpha Actinin
  • Tested applications
    Suitable for: WB, IHC-P, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey, Zebrafish
  • Immunogen

    Recombinant Human Sarcomeric Alpha Actinin.

  • Positive control
    • Human Heart and Skeletal Muscle tissues; A431 and H1299 whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab113086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 104 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC Use at an assay dependent concentration.
ICC/IF 1/100 - 1/200.

Target

  • Function
    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificity
    Expressed in both skeletal and cardiac muscle.
  • Involvement in disease
    Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localization
    Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • Actinin alpha 2 antibody
    • ACTN 2 antibody
    • ACTN2 antibody
    • ACTN2_HUMAN antibody
    • Alpha actinin 2 antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin-2 antibody
    • CMD1AA antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    see all

Anti-Sarcomeric Alpha Actinin antibody images

  • ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry.
  • ab113086 at 10 µg/ml staining Sarcomeric Alpha Actinin in Formalin-fixed, Paraffin-embedded Human Skeletal muscle tissue by Immunohistochemistry.
  • All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab113086) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 104 kDa
  • ab112086 at 1/200 dilution staining Sarcomeric Alpha Actinin in Paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.

References for Anti-Sarcomeric Alpha Actinin antibody (ab113086)

ab113086 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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