Anti-Sarcomeric Alpha Actinin antibody (ab62298)

Overview

  • Product nameAnti-Sarcomeric Alpha Actinin antibody
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit polyclonal to Sarcomeric Alpha Actinin
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 829-878 of Human Sarcomeric Alpha Actinin (EQVIASFRIL ASDKPYILAE ELRRELPPDQ AQYCIKRMPA YSGPGSVPGA)

  • Positive control
    • Fetal heart cell lysate. Human muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab62298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 103 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificityExpressed in both skeletal and cardiac muscle.
  • Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • Actinin alpha 2 antibody
    • ACTN 2 antibody
    • ACTN2 antibody
    • ACTN2_HUMAN antibody
    • Alpha actinin 2 antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin-2 antibody
    • CMD1AA antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    see all

Anti-Sarcomeric Alpha Actinin antibody images

  • Sarcomeric Alpha Actinin antibody (ab62298) at 1.25µg/ml (in 5% skim milk / PBS buffer) + Fetal heart cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 103 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 8%
  • Immunohistochemistry of Human Muscle lysate tissue at an antibody concentration of 4-8µg/ml using anti-ACTN2 antibody (ab62298). The arrows indicate positively stained skeletal muscle cells.

References for Anti-Sarcomeric Alpha Actinin antibody (ab62298)

This product has been referenced in:
  • Maiellaro-Rafferty K  et al. Altered regional cardiac wall mechanics are associated with differential cardiomyocyte calcium handling due to nebulette mutations in preclinical inherited dilated cardiomyopathy. J Mol Cell Cardiol 60:151-60 (2013). WB ; Mouse . Read more (PubMed: 23632046) »
  • Richter W  et al. Conserved expression and functions of PDE4 in rodent and human heart. Basic Res Cardiol 106:249-62 (2011). IHC-Fr ; Human . Read more (PubMed: 21161247) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (cardiac ventricle)
Permeabilization Yes - 0.25% Triton-X
Specification cardiac ventricle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Aug 13 2015

Thank you for your phone call. As we discussed, using more antibody may help to improve your signal. I would recommend trying a dilution of 1:200 with your current protocol. If that does not help to improve your results, I would be happy to offer you a...

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